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The 2021 NFCA Division II Top 25 Poll is voted on by 16 NCAA Division II head coaches with two representing each of the eight NCAA regions. Records reflect games played through April 25. In 2021, universities that are not competing will not be eligible for the poll.
| Rank | Team | Record | Points | Previous |
|---|---|---|---|---|
| 1 | Augustana (12) | 32-4 | 392 | 1 |
| 2 | Valdosta State (3) | 32-3 | 387 | 2 |
| 3 | UT Tyler | 31-5 | 366 | 3 |
| 4 | North Georgia (1) | 33-5 | 356 | 4 |
| 5 | Indianapolis | 34-3 | 332 | 5 |
| 6 | Oklahoma Christian | 33-5 | 316 | 10 |
| 7 | Lincoln Memorial | 36-6 | 306 | 7 |
| 8 | Young Harris | 29-8 | 271 | 9 |
| 9 | East Texas A&M | 25-12 | 264 | 6 |
| 10 | West Texas A&M | 30-10 | 238 | 12 |
| 11 | Auburn Montgomery | 32-5 | 235 | 19 |
| 12 | Concordia | 22-11 | 202 | 11 |
| 13 | Central Oklahoma | 29-9 | 197 | 16 |
| 14 | Grand Valley State | 31-7 | 191 | 14 |
| 15 | Rollins | 16-2 | 178 | 8 |
| 16 | Colorado Mesa | 30-2 | 173 | 17 |
| 17 | Minnesota State | 28-7 | 97 | 18 |
| 18 | UAH | 27-10 | 141 | 13 |
| 19 | Saint Anselm | 20-4 | 136 | 15 |
| 20 | Winona State | 28-7 | 97 | 20 |
| 21 | Southern Arkansas | 20-7 | 68 | 21 |
| 22 | West Florida | 27-12 | 50 | 22 |
| 23 | Angelo State | 29-11 | 44 | 23 |
| 24 | Biola | 19-13 | 38 | |
| 25 | Northwest Nazarene | 24-9 | 18 | |
| Texas A&M-Kingsville | 24-11 | 18 | 24 |
Kutztown (14), Southeastern Oklahoma State (7), Georgian Court (6), Shepherd (5), Central Missouri (1), Tampa (1).
No. 25 Charleston
