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The 2021 NFCA Division II Top 25 Poll is voted on by 16 NCAA Division II head coaches with two representing each of the eight NCAA regions. Records reflect games played through May 2. In 2021, universities that are not competing will not be eligible for the poll.
| Rank | Team | Record | Points | Previous |
|---|---|---|---|---|
| 1 | Augustana (16) | 36-4 | 400 | 1 |
| 2 | UT Tyler | 33-5 | 382 | 3 |
| 3 | North Georgia | 35-5 | 370 | 4 |
| 4 | Auburn Montgomery | 35-5 | 341 | 11 |
| 5 | Lincoln Memorial | 36-6 | 319 | 7 |
| 6 | Oklahoma Christian | 34-6 | 305 | 6 |
| 7 | Indianapolis | 37-5 | 301 | 5 |
| 8 | Valdosta State | 32-6 | 288 | 2 |
| 9 | West Texas A&M | 32-10 | 275 | 10 |
| 10 | Young Harris | 30-9 | 264 | 8 |
| 11 | Concordia | 26-11 | 246 | 12 |
| 12 | East Texas A&M | 25-14 | 213 | 9 |
| 13 | Grand Valley State | 35-7 | 203 | 14 |
| 14 | UAH | 30-10 | 191 | 18 |
| 15 | Central Oklahoma | 32-10 | 177 | 13 |
| 16 | Colorado Mesa | 34-2 | 173 | 16 |
| 17 | Minnesota State | 30-7 | 136 | 17 |
| 18 | Saint Anselm | 24-4 | 124 | 19 |
| 19 | West Florida | 30-12 | 106 | 22 |
| 20 | Winona State | 31-8 | 91 | 20 |
| 21 | Angelo State | 31-11 | 71 | 23 |
| 22 | Southern Arkansas | 23-7 | 68 | 21 |
| 23 | Rollins | 16-2 | 64 | 15 |
| 24 | Georgian Court | 32-4 | 29 | |
| 25 | Biola | 19-13 | 26 | 24 |
Northwest Nazarene (14), Kutztown (11), Texas A&M University-Kingsville (7), Central Missouri (4), Shepherd (1).
t-No. 25 Northwest Nazarene, t-No. 25 Texas A&M-Kingsville
