2000 Louisville Slugger/NFCA Division II All-America Teams
First Team
| Position | Name | School |
| Pitcher | Sarah Van Gorp | Lewis (IL) |
| Pitcher | Julie Fromm | North Dakota State |
| Pitcher | Karissa Hoehn | St. Cloud State (MN) |
| Catcher | Audra Thomas | Kennesaw State (GA) |
| Catcher | Kristi Bredbenner | Truman State (MO) |
| First Base | Kim Biskup | Grand Valley State (MI) |
| First Base | Tracy Jester | North Florida |
| Second Base | Kacey Henden | Mesa State (CO) |
| Third Base | Erin Newman | Southern Illinois-Edwardsville |
| Shortstop | Nikki Flynn-Gregg | North Dakota State |
| Shortstop | Erica Miller | Bloomsburg (PA) |
| Outfield | Jenny Hill | Augustana (SD) |
| Outfield | Kelly Adams | Mesa State (CO) |
| Outfield | Marissa Dugan | Indiana (PA) |
| Designated Player | Jamie Dunn | Mesa State (CO) |
| Utility | Krissy Styrna | West Florida |
| At-Large (C) | Blake Baskin | West Florida |
Second Team
| Position | Name | School |
| Pitcher | Annie Sells |
Armstrong Atlantic State (GA) |
| Pitcher | Melissa Brooks | California (PA) |
| Pitcher | Amanda Kelly | Nebraska-Kearney |
| Pitcher | Melanie Wood | Cal State Bakersfield |
| Catcher | Shelly Rhein | North Dakota State |
| Catcher | Kristine Botto | Massachusetts-Lowell |
| First Base | Julie Phipps | Assumption (MA) |
| Second Base | Melanie Carter | Alabama-Huntsville |
| Third Base | Heather Faria | Merrimack (MA) |
| Shortstop | Audrey Williams | Alabama-Huntsville |
| Outfield | Kelly Moats | Nebraska-Kearney |
| Outfield | Vanessa Sterner | Bloomsburg (PA) |
| Outfield | Chrissa Landwehr | Augustana (SD) |
| Designated Player | Katie Waldo | Southern Illinois-Edwardsville |
| Utility | Michelle Matthews | Mount Olive (NC) |
| At-Large (C) | Shelli Maher |
Humboldt State (CA) |
Third Team
| Position | Name | School |
| Pitcher | Lane Davis | Alabama-Huntsville |
| Pitcher | Jenn Connolly | Merrimack (MA) |
| Pitcher | Angela Lancaster | Columbus State (GA) |
| Catcher | Tiffany Davis | South Dakota |
| Catcher | Patty Thole | Wayne State (MI) |
| First Base | Sharlie Cunningham | UC Davis |
| Second Base | Stephanie Martin | Assumption (MA) |
| Third Base | Elizabeth Card | St. Mary's (TX) |
| Shortstop | Rhonda Matoska | Wisconsin-Parkside |
| Outfield | Shyamala White | UC Davis |
| Outfield | Kate Holden | Pace (NY) |
| Outfield | Beth Bayer | USC Spartanburg |
| Designated Player | Holly Justice | Western New Mexico |
| Utility | Anne Bentley | Ferris State (MI) |
| At-Large (SS) | Leslie Phillips |
East Central (OK) |
